StyrosZyme® Pepsin

Technical Specifications

Support Matrix

Hydrophilic coated cross linked polystyrene/divinyl benzene

Loading

6-8 mg per ml of bed volume

Surface Functionality

Pepsin from Porcine stomach

Shipping solvent

0.02 % sodium azide in DI Water or 10% MeOH in DI Water.

Packing density

0.4 g/ml

Shrinkage/swelling

< 0.5% under different salt or solvent concentrations.

 

General Considerations
Pepsin is an acidic protein with a pI of 1.0. It is unstable above pH 6.9

Below pH 6, pepsin preferentially cleaves on the carboxyl side of L-phenylalanine, L-leucine, or L-tyrosine, where the amino side residue is preferably, but not limited to, an amino acid containing a hydrophobic side chain.
Pepsin is very useful in the preparation of immunoglobulin fragments, specifically from IgG and IgM class antibodies. With IgG antibodies, pepsin will yield F(ab’)2 fragments, whereas with most IgM molecules, it is possible to generate F(ab’)2, Fab, and Fv fragments by using the appropriate conditions.

Enzyme digestion varies with proteins as well as the operating conditions such as temperature, flow rate and pH.

By controlling these variables, it is possible to control the extent of digestion.

StyrosZyme® Pepsin can be ordered in PEEK or Stainless Steel Columns.

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