Facts, News and Frequently Asked Questions

Thanks to you, our loyal and ever-growing customers, we have done it for over 19 consecutive years.
The price of an 8 ml Mono Q or Mono S column is now over $6,000 as compared to under $2,000 for a STYROS® column equivalent.
A 20 ml STYROS® column is still less expensive than a Mono Bead column sold in 1997 (uder $5,000).

Scalability with STYROS® columns is seamless, from 100 mm long 4.6 mm ID column, to 100 mm long 10 mm ID column of similar resolution.

Separation of cell culture with a
4.6 x 100 mm STYROS® HQ/XH column(volume = 1.7 ml)
Flow rate 5 ml/ min (1,800 cm/hr of linear velocity)

Scale up of the same separation of cell culture with a
10 x 100 mm STYROS® HQ/XH column (volume = 8 ml )
Flow rate 5 ml/ min (380 cm/hr of linear velocity)


Separation of mucin free egg white proteins on STYROS® HQ/XH
strong anion exchanger.
(Linear Flow Rate: 360 cm/hr)


Separation of mucin free egg white proteins on Mono Q HR
(Linear Flow Rate: 300 cm/h


See the Application notes for more facts and column performances.

Recent Applications of STYROS® Stationary Phases are listed beleow:

Frequently Asked Questions

Q: What is different with your Polymeric Stationary Phases?
A: Polymeric stationary phases are offered with different degree of cross-linking.
STYROS® products are optimally cross-linked to provide “Hard Gel” that equals the pressure tolerance of Silica without the rigidity and brittleness of Silica.

Q: How high of back pressure can it stand?
A: We have tested it up to 10,000 psi without collapsing the bed.

Q: Are you suggesting that there is no leaching or leakage?
A: Indeed, the column can last many years as the bed remains intact.
Provided that the column is properly used and cleaned after usage in order to avoid the fouling of the bed or the plugging of the frit, the media will not degrade. There is no leaching.

Q: Polystyrene divinylbenzene polymers are hydrophobic, how do you make it hydrophilic? Is there any “shrink-wrapping” involved?
A: No “shrink-wrapping” as the stationary phase is fully porous and therefore fully pervious. Any passive coating such as “shrink-wrapping” would plug the through pores during the passive coating. The coating would naturally leach out during the operation and subsequently create a void.

Q: How are you then coating it?
A: Bonding. Covalent coating using proprietary chemistry. The coating is such that it does not leave any nonspecific patches on the surface of the stationary phase.
The coating neither leaches nor does it plug the through pores.

Q: How do you compare the performance of the media before and after the coating?
A: Considering the orthogonal change in property, the performances compare favorably.

Q: Are there benefits to Polymeric HPLC columns.
A: There are many. The obvious one is the fact that there is no need to functionalize them for reversed-phase separations, and

STYROS® columns are made rigid. They can withstand high back pressures. They are not brittle as Silica and they do not leach.

In addition to these advantages they are offered in anion exchange, cation exchange, HIC, HILIC, Affinity including Protein A.

StyrosZyme® Enzyme Reactor Columns are a line of products that reduce the digestion process of proteins to a few minutes as opposed to hours with no leaching and no enzyme digest contamination. These are also polymer based.

As "Simulated Monolith™" our own proprietary process of manufacturing the media, STYROS ® offers the added advantage of low back pressures while maintining high resolution similar to Monoliths.