SIMULATED MONOLITH™

STYROSä HIC-t-Butyl,  SIMULATED MONOLITH™ columns for High Speed, High Performance Liquid Chromatography.

This product was added to our previous set of HIC products at the request of a costumer who was looking for a bulkier group than our STYROS™ HIC Butyl phase.

STYROSä HIC-t-Butyl provides the same high chromatographic performance and chemical stability and is also provided with the same versatility and cost as the other  SIMULATED MONOLITH™ columns.

Standard Separation of 6 proteins  on STYROS™ HIC-t-Butyl/XH

 4.6 x 100 mm column. 2 ml/min (720 cm/hr)

Contents:

• Product Summary 
• Key Features
• Specifications

• Product summary: 

STYROS™ HIC series are a new line of Simulated Monolithä packed columns made of polymeric media intended for the chromatography of biomolecules such as proteins and peptides.

The base monolithic matrix that is fully pervious is made of highly crosslinked poly (styrene-divinylbenzene).

The unique macroporous structure of the bed provides a maximum surface contact as well as a uniform flow path, making it possible to run high speed, high-resolution separations without any mass transfer restrictions.

Unlike soft gel, Simulated Monolith™ columns can be used at high backpressures, reducing considerably the run time.

Typical linear flow rates are 1,800 cm/hr or above, compared to 140 cm/hr with soft gel.

Unlike Monolith, there are no limitations with Simulated Monolith™ columns. They are available in most sizes and configurations.

Higher practical and efficient use of the column is another advantage of such products.

HIC is the method of choice for the purification and separation of biomolecules that have been precipitated with ammonium sulfate or eluted in high salt during ion exchange separations.

Compared to reverse phase, HIC provides a milder technique in using the surface hydrophobicity of biomolecules for their separations. Since no organic solvent is involved, the molecules retain their biological activity.

• Key Features:

Feature 1:

Optimum protein capacity of up to 35 mg/ml 

Feature 2:

Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the beads, we recommend the following pressure limits:

4,000 psi for the XH series,

3,000 psi for the XP and XM series.

4,000 psi for the NB series 

4,000 psi for the MB series

Feature 3:

No measurable shrinking and/or swelling due to highly crosslinked nature of the beads.

• Specifications:

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