FYI NOTE

HPLC System.

HP 1100, standard cell.

Column

STYROS™ 2R/XH
50 x 4.6mm

Mobile Phase

A: 0.1% TFA in H2O
B: 0.1% TFA in Acetonitrile/H2O (95/5)(v/v)

Flow rate

2.5 ml/min (900cm/hr)

Gradient

15 to 80% B in 6.25 Column Volume

Temperature

Ambient

Detection

214 nm

Injection volume

20 ml

Sample
(1 mg/ml each)

1-  99% pure BSA.
2-  99% pure Ovalbumin

HPLC System.

HP 1100, standard cell.

Column

STYROS™ Q  /NB
250 x 2.1mm

Mobile Phase

A: 20 mM Tris , pH= 8.2
B: A + 1 M NaCl, pH= 8.2

Flow rate

2 ml/min (3,470 cm/hr)

Gradient

10% B to 40% B in 31 Column Volume

Temperature

Ambient

Detection

280 nm

Injection volume

5 ml

Sample
(5 mg/ml each)

1,2,3,4,5-: Components of a 99% pure sample of Ovalbumin.
6,7- : Components of a 99 % pure sample of BSA.