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Cost, lifespan and leaching  were considered in the development of this new Affinity chromatography product.

Capture of IgG from a mixture of HSA and IgG run at 5 ml/min
(1,800 cm/hr) on a 4.6 x 33 mm STYROS™ rA column.

 

Capture of IgG from a mixture of HSA and IgG run at 2 ml/min
(3,600 cm/hr) on a 2.1 x 33 mm STYROS™ rA column.

Contents:

  • Product Summary 
  • Key Features
  • Specifications

  • Product summary: 

STYROSä rA (Immobilized Recombinant Protein A) are the first Simulated Monolithä packed columns made of polymeric media intended for analytical and preparative affinity purification of antibodies.

The base monolithic matrix that is fully pervious is made of highly crosslinked poly (styrene-divinylbenzene).

The unique macroporous structure of the bed provides maximum surface contact as well as uniform flow path, making it possible to run high speed, high-resolution captures/separations without any mass transfer restrictions.

Unlike soft gel, Simulated Monolithä columns can be used at high backpressures, reducing considerably the run time.

Typical linear flow rates are 1,800 cm/hr and above, compared to 140 cm/hr with soft gel.

Unlike Monolith, there are no limitations with Simulated Monolithä columns. They are available in most sizes and configurations.

Higher practical speed and efficient use of the column is another advantage of such products.

STYROSä rA media are available in different sizes.

 

  • Key Features:

Feature 1:

Dynamic binding capacity @ 3,600 cm/hr:  35-40  mg/ml.

Feature 2:

Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the packed columns, we recommend that the pressure be kept under 2,000 psi during the loading and unloading of the columns.

Feature 3:

No measurable shrinking and/or swelling due to highly crosslinked nature of the beads.

 

  • Specifications:

Support Matrix Crosslinked Poly(styrene-divinylbenzene) 
Surface Functionalities   Tethered recombinant protein A
Binding Capacity 35-40 mg/ml Human IgG @ pH=7.5
Shipping Solvent 10 % MeOH in DI Water
Packing Density 0.4 g/ml
Shrinkage/Swelling < 0.2 % under different salt or solvent concentrations
Maximum Pressure Tolerance 140 bar (2,000 psi,  14 MPa)

 

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