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High
Capacity Strong and Weak Anion Exchanger
STYROS™ HQ and
STYROS™ HPA
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STYROS™
HQ/NB
Polymeric Porous
hard gel
2.1 x 150 mm.
(No length limit for column )
Separation of egg white proteins at 900 cm/hr (0.5 ml/min).
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To
compare with:
Monodispersed soft gel
5 x 50 mm.
(Column length limited)
Separation of egg white proteins at 300 cm/hr
(1 ml/min).
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Contents:
- Product Summary
- Key Features
- Specifications
STYROS™ HQ and
STYROS™ HPA are the High Capacity versions of the previously
developed anion exchangers for High Throughput purification of proteins and
peptides.
The base matrix that is fully pervious is made of highly crosslinked
poly(styrene-divinylbenzen).
Our proprietary technique ensures that the beads are not brittle and as a
result, free of any leachables.
The unique macroporous structure takes full advantage of the inner bead's
surface area making it possible to run high speed, high resolution separation
without any mass transfer restriction.
STYROS™ HQ and HPA provide
high static and dynamic capacities for analytical and semi preparative
separations.
They have extremely high pressure tolerance, and can be used up to 5,000 psi.
High resolution separation can be achieved at high, as well as low flow rates.
The goal has been to provide seamless series of stationary phases that could
facilitate the transition from analytical to semi preparative and preparative
applications, all within the same products.
Our proprietary column packing are geared towards providing high batch to batch
reproducibility.
Feature 1:
High protein capacity (measured with BSA)
90 mg/ml for STYROS™ HQ and
80 mg/ml for STYROS™ PA
Feature 2:
Although we have used pressures of up to 10,000 psi
without irreversibly altering the structure of the beads, we recommend the
following pressure limits:
4,000 psi for the XH series,
3,000 psi for the XP and XM series.
4,000 psi for the NB series
4,000 psi for the MB series
Feature 3:
No measurable shrinking and/or swelling due to highly
crosslinked nature of the beads.
| Support Matrix |
Crosslinked
Poly(styrene-divinylbenzene) |
Surface Functionalities
STYROS™ HQ
STYROS™ HPA |
Quaternary Aminomethyl
Polyamine |
| Shipping Solvent |
30 % MeOH in DI Water |
| Packing Density |
0.4 g/ml |
| Shrinkage/Swelling |
< 0.5 % under different salt or solvent concentrations |
| Maximum Pressure Tolerance |
270 bar (4,000 psi, 27 MPa) |
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