STYROS® HIC (Hydrophobic Interaction Chromatography)
As Simulated Monolith™ fully uniform polymer based packings, they are intended for use in high speed, high resolution applications.
The base matrix that is fully pervious is made of highly cross linked poly (styrene-divinylbenzene).
It has a high pressure tolerance, and can be used routinely up to 3,000 psi.
The goal has been to provide seamless series of stationary phases that could facilitate the transition from analytical to semi preparative and preparative applications, all within the same products and with high reproducibility.
HIC is the method of choice for the purification and separation of biomolecules that have been precipitated with ammonium sulfate or eluted in high salt during ion exchange separations.
Compared to reverse phase, HIC provides a milder technique in using the surface hydrophobicity of biomolecules for their separations.
Since no organic solvent is involved, the molecules retain their biological activity.
STYROS® HIC are provided in four different types in order to accommodate most applications: